
Our work has laid a foundation for future research on biofilms on Laboulbeniomycetes. We suggest genetics and metabolomics approaches to investigate possible interactions between Laboulbeniales fungi and the biofilms. These bacterial communities were mainly found on the thalli, and only in small quantities on the arthropod integument. associated with Harmonia axyridis (Coccinellidae). flagellata associated with Limodromus assimilis (Carabidae), and Hesperomyces virescens s.l. Scanning electron microscopy micrographs show an abundance of bacterial biofilms on thalli of three species: Laboulbenia collae associated with Paranchus albipes (Carabidae), L. This study is the first to show the presence of biofilms on thallus surfaces of Laboulbeniales, an order of fungi that have biotrophic associations with arthropod hosts. These microbial communities are of interest both from an ecological and a biotechnological point of view, as they have been shown to play a role in biodegradation and biosynthesis. bisporus strains.īacterial biofilms have been described on a number of fungal taxa. Typically, 3-6% of primordia developed into mature sporophores, but significant differences in this proportion, as well as in the numbers of primordia produced, were recorded between 12 A. In larger-scale, nonaxenic culture, strain B430 produced severely malformed but mature sporophores in similar numbers to those of other strains. However, none of these rudimentary primordia developed differentiated tissues or beyond 4 mm diameter, either on axenic casing material in the microcosms or in larger-scale culture. Of six strains tested, only the developmental variant mutant, B430, produced rudimentary primordia on axenic peat-based casing material. bisporus (commercial strain A15) was capable of producing primordia and mature sporophores on charcoal (wood and activated), anthracite coal, lignite and zeolite, but not on bark, coir, peat, rockwool, silica or vermiculite. bisporus were cultured in axenic and nonaxenic microcosms, using a rye grain substrate covered by a range of organic and inorganic casing materials. Some of these primordia then may develop further into sporophores, involving differentiation of tissue. The mushroom (Agaricus bisporus) has a requirement for a "casing layer" that has specific physical, chemical and microbiological properties which stimulate and promote the initiation of primordia.
